Difference between revisions of "Part:BBa C0012"

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[[Image:LacRep-black.jpg]]
 
[[Image:LacRep-black.jpg]]
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===Usage and Biology===
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This particular LacI protein is derived from e.coli and contributed by Michael Elowitz (see Part Design for more information)

Revision as of 19:26, 5 July 2006

lacI repressor from E. coli (+LVA)

Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator BBa_R0010 and PLlac01 hybrid regulator BBa_R0011 and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription.

A rapid degradation tail (LVA) has been added to improve the High to Low performance of this part.

LacRep-black.jpg

Usage and Biology

This particular LacI protein is derived from e.coli and contributed by Michael Elowitz (see Part Design for more information)