Difference between revisions of "Part:BBa K1159007"

Revision as of 23:00, 15 September 2013

Secretory Laccase (IgKappa-SigP_Laccase) in RFC[25] N-Part

Laccase from Bacillus pumilus, which degrades a wide variety of phenolic and non-phenolic compounds, is fused with a N-terminal signal peptide for secretion (Ig Kappa signal peptide from Mus musculus) with also works in moss (Physcomitrella patens). This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 193
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1159007 short</partinfo>
-Laccase from ''Bacillus pumilus'' is fused with a N-terminal signal peptide for secretion (Ig Kappa signal peptide from ''Mus musculus'') with also works in moss (''Physcomitrella patens''). This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.
+Laccase from ''Bacillus pumilus'', which degrades a wide variety of phenolic and non-phenolic compounds, is fused with a N-terminal signal peptide for secretion (Ig Kappa signal peptide from ''Mus musculus'') with also works in moss (''Physcomitrella patens''). This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.
 <!-- Add more about the biology of this part here