Difference between revisions of "Part:BBa K1149004"

Revision as of 19:14, 15 September 2013

pelB-pueB expression

PUR degradation enzyme from

references:

[http://www.sciencedirect.com/science/article/pii/S0964830501000427 Cloning, nucleotide sequencing and characterization of a polyurethanase gene (pueB) from Pseudomonas chlororaphis (2001)] <p/> <p> [http://www.sciencedirect.com/science/article/pii/S0964830502000690 secretion and expression in E.coli]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 99
Illegal BglII site found at 1213
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 407
Illegal AgeI site found at 752
Illegal AgeI site found at 1241
Illegal AgeI site found at 1517
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1149004 short</partinfo>
-PUR degradation
+PUR degradation enzyme from
+https://static.igem.org/mediawiki/parts/3/31/PUR_esterase_theoretical_pathway_2.jpg
+<p>references: </p>
+<p> [http://www.sciencedirect.com/science/article/pii/S0964830501000427 Cloning, nucleotide sequencing and characterization of a polyurethanase gene (pueB) from Pseudomonas chlororaphis (2001)] <p/>
+<p> [http://www.sciencedirect.com/science/article/pii/S0964830502000690 secretion and expression in E.coli] </P>
 <!-- Add more about the biology of this part here