Difference between revisions of "Part:BBa K1149005"

Revision as of 19:09, 15 September 2013

pelB-pudA expression

This part can express a plastic degradation enzyme from Comamonas acidovorans that breaks down ester bonds in polyurethane. It containes a Gly-X-Ser-X-Gly motif characteristic of serine hydrolases. The sequence was codon optimized for E.coli. We added a pelB tag to ensure secretion to the N terminal and we have added a 6xpolyhistidine tag to the C terminal. The promoter is arabinose inducible.

Theoretical pathway of PUR degradation by esterase:

references:

[http://www.selu.edu/acad_research/depts/biol/faculty/publications/pdf/2012/howard2012.pdf Howard, Gary 2012 pg:371 -3, Polyurethane Biodegradation, Environmental Science]

[http://www.sciencedirect.com/science/article/pii/S0922338X99890011 Cloning and sequence analysis of a polyurethane esterase of Comamonas acidovorans 1998]

[http://www.sciencedirect.com/science/article/pii/S0964830598000663 Purification and characterization of a soluble polyurethane degrading enzyme from Comamonas acidovorans 1999]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 125
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 65
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 529
Illegal AgeI site found at 604
Illegal AgeI site found at 1753
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1149005 short</partinfo>
-This part can express a plastic degradation enzyme from Comamonas acidovorans that breaks down ester bonds in polyurethane. The sequence was codon optimized for E.coli. We added a pelB tag to ensure secretion to the N terminal and we have added a 6xpolyhistidine tag to the C terminal.
+This part can express a plastic degradation enzyme from Comamonas acidovorans that breaks down ester bonds in polyurethane. It containes a Gly-X-Ser-X-Gly motif characteristic of serine hydrolases. The sequence was codon optimized for E.coli. We added a pelB tag to ensure secretion to the N terminal and we have added a 6xpolyhistidine tag to the C terminal.
 The promoter is arabinose inducible.