Difference between revisions of "Part:BBa K1217000"

 
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<partinfo>BBa_K1217000 short</partinfo>
 
<partinfo>BBa_K1217000 short</partinfo>
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===Usage and Biology===
 
===Usage and Biology===
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Testing
  
 
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Revision as of 18:56, 15 September 2013

pT7-ppk1(T.forsythia)

Polyphosphate kinase (ppk1) (E.C. 2.7.4.1) catalyzes the formation of polyphosphate from ATP. In BBa_K1217000, ppk1 was under the control of T7 promotor. Under iptg induction, over-expression of ppk1 can reduce the phosphate level in the medium by its incorporation into polyphosphate synthesis inside the bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 364
    Illegal BglII site found at 1826
    Illegal XhoI site found at 108
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1284
    Illegal NgoMIV site found at 1596
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 384