Difference between revisions of "Part:BBa K1149022"

Revision as of 18:23, 15 September 2013

pelB (E.coli optimized)

N terminal tag that secretes proteins in E.coli when attached to N terminus. It is an E.coli codon optimised version of pelB tag BBa_K208004 or BBa_J32015. It only differs from these two in codon use but has the exact same amino acid sequence.

Please look at the information on these pages in order to find out more about pelB and about proteins that have been previously secreted using this strategy.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1149022 short</partinfo>
-secreted protein when attached to N terminus
+N terminal tag that secretes proteins in E.coli when attached to N terminus.
-It is an E.coli codon optimised version of pelB tag [https://parts.igem.org/wiki/index.php?title=Part:BBa_K208004 BBa_K208004].
+It is an E.coli codon optimised version of pelB tag [https://parts.igem.org/wiki/index.php?title=Part:BBa_K208004 BBa_K208004] or [https://parts.igem.org/Part:BBa_J32015 BBa_J32015]. It only differs from these two in codon use but has the exact same amino acid sequence.
+Please look at the information on these pages in order to find out more about pelB and about proteins that have been previously secreted using this strategy.
 <!-- Add more about the biology of this part here