Difference between revisions of "Part:BBa R0080"
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<partinfo>BBa_R0080 short</partinfo>
 
<partinfo>BBa_R0080 short</partinfo>
  
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[http://openwetware.org/wiki/Arabinose AraC] operator, truncated to include araO1, araI1, araI2, c-amp1, and c-amp2 sites.  This operator should *activate* transcription in the presence of AraC; b/c the operator lacks the araO2 site, there should not be araC-mediated repression.
+
AraC operator, truncated to include araO1, araI1, araI2, c-amp1, and c-amp2 sites.  This operator should *activate* transcription in the presence of AraC or the simple sugar [http://openwetware.org/wiki/Arabinose arabinose]
 +
 
 +
b/c the operator lacks the araO2 site, there should not be araC-mediated repression.
  
  

Revision as of 01:01, 4 July 2006

Promoter (AraC regulated)

AraC operator, truncated to include araO1, araI1, araI2, c-amp1, and c-amp2 sites. This operator should *activate* transcription in the presence of AraC or the simple sugar [http://openwetware.org/wiki/Arabinose arabinose]

b/c the operator lacks the araO2 site, there should not be araC-mediated repression.


Usage and Biology

araC binding region from E.coli


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 103
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]