Difference between revisions of "Part:BBa K1159100"

Revision as of 15:30, 15 September 2013

Autolysis resistant TEV Protease S219V mutant in RFC[25]

TEV Protease from Tabacco Etch Virus (TEV) is a highly specific protease that cleaves the sequence "Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) (ENLYFQ(G/S))" very specifically before the last Gly/Ser. The wildtype version of the protease has a short half-life due to self-inactivation through autolysis. This mutant S219V is autolysis resistant and its enzymatic activity is not affected by the mutation. This part is flanked by RFC[25] pre- and suffix for further protein fusions.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 316
Illegal SapI.rc site found at 664

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1159100 short</partinfo>
-TEV Protease from Tabacco Etch Virus (TEV) is a highly specific protease that cleaves the sequence "Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) (ENLYFQ(G/S))" very specifically before the last Gly/Ser. The wildtype version of the protease has a short half-life due to self-inactivation through autolysis. This mutant S219V is autolysis resistant and its enzymatic activity is not affected by the mutation.
+TEV Protease from Tabacco Etch Virus (TEV) is a highly specific protease that cleaves the sequence "Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) (ENLYFQ(G/S))" very specifically before the last Gly/Ser. The wildtype version of the protease has a short half-life due to self-inactivation through autolysis. This mutant S219V is autolysis resistant and its enzymatic activity is not affected by the mutation. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
 <!-- Add more about the biology of this part here