Difference between revisions of "Part:BBa K1149007"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1149007 short</partinfo> | <partinfo>BBa_K1149007 short</partinfo> | ||
Encodes a peptidase from proteinase K family, breaks down polylactic acid. | Encodes a peptidase from proteinase K family, breaks down polylactic acid. | ||
− | The BBa_K1149008 construct is the same enzyme but wit the pelB secretion tag. | + | The [https://parts.igem.org/Part:BBa_K1149008 BBa_K1149008] construct is the same enzyme but wit the pelB secretion tag. |
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+ | <p>.</p> | ||
+ | reference: Larsen AN, Moe E, Helland R, Gjellesvik DR, Willassen NP.Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia sp. FEBS J. 2006 Jan;273(1):47-60. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 14:55, 15 September 2013
Proteinase K
Encodes a peptidase from proteinase K family, breaks down polylactic acid. The BBa_K1149008 construct is the same enzyme but wit the pelB secretion tag.
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reference: Larsen AN, Moe E, Helland R, Gjellesvik DR, Willassen NP.Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia sp. FEBS J. 2006 Jan;273(1):47-60.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
Illegal NheI site found at 943 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 290
Illegal BamHI site found at 65 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1465
- 1000COMPATIBLE WITH RFC[1000]