Difference between revisions of "Part:BBa J24666"

Revision as of 17:03, 3 July 2006

Toggle Switch to Immobilize E. Coli

This part uses a pLac promoter to create motB and also uses an inverter (cI and pcI) to produce LacI. In addition LasI is produced in the motile state, which creates the signalling molecule AI-1. LasR is a transcription factor created in the immobile state. AI-1 and LasR form a complex to initiate transcription from pLasR promoter. We call this the Freeze Machine.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 886
Illegal BglII site found at 4006
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 4361
Illegal AgeI site found at 448
Illegal AgeI site found at 4558
Illegal AgeI site found at 4945
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1220

@@ Line 2: / Line 2: @@
 <partinfo>BBa_J24666 short</partinfo>
-This part uses a pLac promoter to create motB and also uses an inverter (cI and pcI) to produce LacI.  In addition LasI is produced in the motile state, which creates the signalling molecule AI-1.  LasR is a transcription factor created in the immobile state.  AI-1 and LasR form a complex to initiate transcription from pLasR promoter.  We call this the Freeze Machine.
+This part uses a pLac promoter to create motB and also uses an inverter (cI and pcI) to produce LacI.  In addition LasI is produced in the motile state, which creates the signalling molecule AI-1.  LasR is a transcription factor created in the immobile state.  AI-1 and LasR form a complex to initiate transcription from pLasR promoter.  We call this the '''''Freeze Machine.'''''
 [[Image:BrownFreezeMachine.jpg]]