Difference between revisions of "Part:BBa J24666"
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This part uses a pLac promoter to create motB and also uses an inverter (cI and pcI) to produce LacI. In addition LasI is produced in the motile state, which creates the signalling molecule AI-1. LasR is a transcription factor created in the immobile state. AI-1 and LasR form a complex to initiate transcription from pLasR promoter. We call this the Freeze Machine. | This part uses a pLac promoter to create motB and also uses an inverter (cI and pcI) to produce LacI. In addition LasI is produced in the motile state, which creates the signalling molecule AI-1. LasR is a transcription factor created in the immobile state. AI-1 and LasR form a complex to initiate transcription from pLasR promoter. We call this the Freeze Machine. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 17:02, 3 July 2006
Toggle Switch to Immobilize E. Coli
This part uses a pLac promoter to create motB and also uses an inverter (cI and pcI) to produce LacI. In addition LasI is produced in the motile state, which creates the signalling molecule AI-1. LasR is a transcription factor created in the immobile state. AI-1 and LasR form a complex to initiate transcription from pLasR promoter. We call this the Freeze Machine.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 886
Illegal BglII site found at 4006 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4361
Illegal AgeI site found at 448
Illegal AgeI site found at 4558
Illegal AgeI site found at 4945 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1220