Difference between revisions of "Part:BBa K1062000"

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2) Improve past circuits with better decision making by using dCas9 instead of traditional repressors.
 
2) Improve past circuits with better decision making by using dCas9 instead of traditional repressors.
  
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=='''Conjugation Project'''==
  
 
[[File:Conjugation project.jpg]]
 
[[File:Conjugation project.jpg]]
  
 +
=='''Synthetic Circuit'''==
  
 
[[File:Synthetic Circuit.jpg]]
 
[[File:Synthetic Circuit.jpg]]
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=='''Conjugation Project'''==
 
−
 
−
=='''Synthetic Circuit'''==
 
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 18:02, 13 September 2013

Guide RNA (gRNA) target for RFP

This part is a guide RNA (gRNA) that targets RFP, when bound to Cas9. It also contains a cleavage site recognized by the Csy4 enzyme which allows it to be cleaved from neighboring DNA/RNA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

In both of our projects, we used gRNAs and dCas9 as: 1) To specifically target and repress the "virulence gene" (we used flourescent proteins as a proof of concept) in our conjugation project 2) Improve past circuits with better decision making by using dCas9 instead of traditional repressors.

Conjugation Project

Conjugation project.jpg

Synthetic Circuit

Synthetic Circuit.jpg