Difference between revisions of "Part:BBa K1062000"
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1) To specifically target and repress the "virulence gene" (we used flourescent proteins as a proof of concept) in our conjugation project | 1) To specifically target and repress the "virulence gene" (we used flourescent proteins as a proof of concept) in our conjugation project | ||
2) Improve past circuits with better decision making by using dCas9 instead of traditional repressors. | 2) Improve past circuits with better decision making by using dCas9 instead of traditional repressors. | ||
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[[File:Conjugation project.jpg]] | [[File:Conjugation project.jpg]] | ||
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| + | [[File:Synthetic Circuit.jpg]] | ||
=='''Conjugation Project'''== | =='''Conjugation Project'''== | ||
Revision as of 17:47, 13 September 2013
Guide RNA (gRNA) target for RFP
This part is a guide RNA (gRNA) that targets RFP, when bound to Cas9. It also contains a cleavage site recognized by the Csy4 enzyme which allows it to be cleaved from neighboring DNA/RNA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
In both of our projects, we used gRNAs and dCas9 as: 1) To specifically target and repress the "virulence gene" (we used flourescent proteins as a proof of concept) in our conjugation project 2) Improve past circuits with better decision making by using dCas9 instead of traditional repressors.
