Difference between revisions of "Part:BBa K1062000"
(→Usage and Biology) |
(→Usage and Biology) |
||
| Line 14: | Line 14: | ||
1) To specifically target and repress the "virulence gene" (we used flourescent proteins as a proof of concept) in our conjugation project | 1) To specifically target and repress the "virulence gene" (we used flourescent proteins as a proof of concept) in our conjugation project | ||
2) Improve past circuits with better decision making by using dCas9 instead of traditional repressors. | 2) Improve past circuits with better decision making by using dCas9 instead of traditional repressors. | ||
| + | |||
| + | [[File:Conjugation project.jpg]] | ||
=='''Conjugation Project'''== | =='''Conjugation Project'''== | ||
Revision as of 17:25, 13 September 2013
Guide RNA (gRNA) target for RFP
This part is a guide RNA (gRNA) that targets RFP, when bound to Cas9. It also contains a cleavage site recognized by the Csy4 enzyme which allows it to be cleaved from neighboring DNA/RNA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
In both of our projects, we used gRNAs and dCas9 as: 1) To specifically target and repress the "virulence gene" (we used flourescent proteins as a proof of concept) in our conjugation project 2) Improve past circuits with better decision making by using dCas9 instead of traditional repressors.
