Difference between revisions of "Part:BBa J23006:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J23006 short</partinfo>
 
<partinfo>BBa_J23006 short</partinfo>
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===Design Notes===
 
===Design Notes===
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N/A
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Effectively, this plasmid is pSB1A3 with:
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# b0015.r0040 inserted between the EcoRI and XbaI sites
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# r0040.b0034.E1010.b0015 [OnRFP] inserted between the XbaI and SpeI sites
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Revision as of 01:51, 2 July 2006

Part-making plasmid with Ptet-XbaI-[biobrick]-SpeI-PstI


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Illegal EcoRI site found at 2136
    Illegal XbaI site found at 2350
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2136
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2142
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2136
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Illegal suffix found at 2
    Illegal EcoRI site found at 2136
    Illegal XbaI site found at 2350
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2136
    Illegal XbaI site found at 2350
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1175


Design Notes

Effectively, this plasmid is pSB1A3 with:

  1. b0015.r0040 inserted between the EcoRI and XbaI sites
  2. r0040.b0034.E1010.b0015 [OnRFP] inserted between the XbaI and SpeI sites



Source

pSB1A2-I13521 See KAD062806-Construction of J23006 for details

References