Difference between revisions of "Part:BBa K1062000"

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<partinfo>BBa_K1062000 short</partinfo>
 
<partinfo>BBa_K1062000 short</partinfo>
  
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This part is a guide RNA (gRNA) that targets RFP, when bound to Cas9. It also contains a cleavage site recognized by the Csy4 enzyme which allows it to be cleaved from neighboring DNA/RNA.  
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This part is a guide RNA (gRNA) that targets RFP, when bound to Cas9. It also contains a cleavage site recognized by the Csy4 enzyme which allows it to be cleaved from neighboring DNA/RNA.
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<!-- Uncomment this to enable Functional Parameter display
 
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===Functional Parameters===
 
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<partinfo>BBa_K1062000 parameters</partinfo>
 
 
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1062000 SequenceAndFeatures</partinfo>
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==='''Usage and Biology'''===
 
==='''Usage and Biology'''===
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1) To specifically target and repress the "virulence gene" (we used flourescent proteins as a proof of concept) in our conjugation project  
 
1) To specifically target and repress the "virulence gene" (we used flourescent proteins as a proof of concept) in our conjugation project  
 
2) Improve past circuits with better decision making by using dCas9 instead of traditional repressors.
 
2) Improve past circuits with better decision making by using dCas9 instead of traditional repressors.
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K1062000 parameters</partinfo>
 
<!-- -->
 
<!-- -->
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K1062000 SequenceAndFeatures</partinfo>
 

Revision as of 17:02, 13 September 2013

Guide RNA (gRNA) target for RFP

This part is a guide RNA (gRNA) that targets RFP, when bound to Cas9. It also contains a cleavage site recognized by the Csy4 enzyme which allows it to be cleaved from neighboring DNA/RNA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

In both of our projects, we used gRNAs and dCas9 as: 1) To specifically target and repress the "virulence gene" (we used flourescent proteins as a proof of concept) in our conjugation project 2) Improve past circuits with better decision making by using dCas9 instead of traditional repressors.