Difference between revisions of "Part:BBa K1116002:Experience"

(Applications of BBa_K1116002)
(Applications of BBa_K1116002)
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By testing the mean value of EYFP (Enhance Yellow Fluorescent Protein) and the relationship between the EYFP and the DNA copy number, we can know whether the plasmids work or not.
 
By testing the mean value of EYFP (Enhance Yellow Fluorescent Protein) and the relationship between the EYFP and the DNA copy number, we can know whether the plasmids work or not.
 
In our design, we use mkate (another fluorescent protein), which radiats red lights, as reference gene to reflect copy number. The results as follows. We can distinguish the mean valve of EYFP of circuit A and circuit B from the Figure C.
 
In our design, we use mkate (another fluorescent protein), which radiats red lights, as reference gene to reflect copy number. The results as follows. We can distinguish the mean valve of EYFP of circuit A and circuit B from the Figure C.
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[[File:Figure C.jpg]]
  
 
===User Reviews===
 
===User Reviews===

Revision as of 08:52, 13 September 2013


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1116002

In our project, BBa_K1116002 serves as an auxiliary node. Our design as follows (Circuit A). Besides, BBa_K1116003 is used in our control design (Circuit B). The difference between BBa_K1116002 and BBa_K1116003 is the T21 targets.

Figure A.jpg Figure B.jpg

By testing the mean value of EYFP (Enhance Yellow Fluorescent Protein) and the relationship between the EYFP and the DNA copy number, we can know whether the plasmids work or not. In our design, we use mkate (another fluorescent protein), which radiats red lights, as reference gene to reflect copy number. The results as follows. We can distinguish the mean valve of EYFP of circuit A and circuit B from the Figure C.

Figure C.jpg

User Reviews

UNIQa70c320624ea5e17-partinfo-00000000-QINU UNIQa70c320624ea5e17-partinfo-00000001-QINU