Difference between revisions of "Part:BBa K1116002:Experience"
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CubicStone (Talk | contribs) (→Applications of BBa_K1116002) |
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===Applications of BBa_K1116002=== | ===Applications of BBa_K1116002=== | ||
| − | In our project, BBa_K1116002 serves as an auxiliary node. Our design as follows ( | + | In our project, BBa_K1116002 serves as an auxiliary node. Our design as follows (Circuit A). Besides, BBa_K1116003 is used in our control design (Circuit B). The difference between BBa_K1116002 and BBa_K1116003 is the T21 targets. |
[[File:Figure A.jpg]] [[File:Figure B.jpg]] | [[File:Figure A.jpg]] [[File:Figure B.jpg]] | ||
| + | |||
| + | By testing the mean value of EYFP (Enhance Yellow Fluorescent Protein) and the relationship between the EYFP and the DNA copy number, we can know whether the plasmids work or not. | ||
| + | In our design, we use mkate (another fluorescent protein), which radiats red lights, as reference gene to reflect copy number. The results as follows. We can distinguish the mean valve of EYFP of circuit A and circuit B from the Figure C. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 08:42, 13 September 2013
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how you used this part and how it worked out.
Applications of BBa_K1116002
In our project, BBa_K1116002 serves as an auxiliary node. Our design as follows (Circuit A). Besides, BBa_K1116003 is used in our control design (Circuit B). The difference between BBa_K1116002 and BBa_K1116003 is the T21 targets.
By testing the mean value of EYFP (Enhance Yellow Fluorescent Protein) and the relationship between the EYFP and the DNA copy number, we can know whether the plasmids work or not. In our design, we use mkate (another fluorescent protein), which radiats red lights, as reference gene to reflect copy number. The results as follows. We can distinguish the mean valve of EYFP of circuit A and circuit B from the Figure C.
User Reviews
UNIQ03590aab23be5d7e-partinfo-00000000-QINU UNIQ03590aab23be5d7e-partinfo-00000001-QINU