Difference between revisions of "Part:BBa K1114512"

Revision as of 03:13, 13 September 2013

Level 1 MoClo device that constitutively expresses araC

pJ04B2C80_EF

This is a Level 1 MoClo device that constitutively expresses araC It contains the Level 0 parts J23104_EB, BCD2_BC, C0080_CD, and B0015_DF within the DVL1_EF backbone. In the given sequence the internal fusion sites are incorrectly duplicated. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 11
Illegal NheI site found at 34
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1114512 short</partinfo>
 <html>
-pJ04B2C12_EF
+pJ04B2C80_EF<br><br>
-This is a Level 1 <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> device that constitutively expresses the repressor lacI. It contains the Level 0 parts <a href="https://parts.igem.org/Part:BBa_K1114006">J23104_EB</a>, <a href="https://parts.igem.org/Part:BBa_K1114107:Design">BCD2_BC</a>, <a href="https://parts.igem.org/Part:BBa_K1114204">C0080_CD</a>, and <a href="https://parts.igem.org/Part:BBA_K783060">B0015_DF</a> within the <a href="https://parts.igem.org/Part:BBa_K1114419">DVL1_EF</a> backbone. In the given sequence the internal fusion sites are incorrectly duplicated.
+This is a Level 1 <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> device that constitutively expresses araC It contains the Level 0 parts <a href="https://parts.igem.org/Part:BBa_K1114006">J23104_EB</a>, <a href="https://parts.igem.org/Part:BBa_K1114107:Design">BCD2_BC</a>, <a href="https://parts.igem.org/Part:BBa_K1114204">C0080_CD</a>, and <a href="https://parts.igem.org/Part:BBA_K783060">B0015_DF</a> within the <a href="https://parts.igem.org/Part:BBa_K1114419">DVL1_EF</a> backbone. In the given sequence the internal fusion sites are incorrectly duplicated.
 The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.