Difference between revisions of "Part:BBa K1114514:Design"

 
(→‎Design Notes)
 
Line 10: Line 10:
 
pJ04B2CY_EF
 
pJ04B2CY_EF
  
−
This is a Level 1 <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> device that constitutively expresses the reporter YFP. It contains the Level 0 parts <a href="https://parts.igem.org/Part:BBa_K1114006">J23104_EB</a>, <a href="https://parts.igem.org/Part:BBa_K1114107:Design">BCD2_BC</a>, <a href="https://parts.igem.org/Part:BBa_K1114208">E0030_CD</a>, and <a href="https://parts.igem.org/Part:BBA_K783060">B0015_DF</a> within the <a href="https://parts.igem.org/Part:BBa_K1114419">DVL1_EF</a> backbone. In the given sequence the internal fusion sites are incorrectly duplicated.
+
This is a Level 1 <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> device that constitutively expresses the reporter YFP. It contains the Level 0 parts <a href="https://parts.igem.org/Part:BBa_K1114006">J23104_EB</a>, <a href="https://parts.igem.org/Part:BBa_K1114107:Design">BCD2_BC</a>, <a href="https://parts.igem.org/Part:BBa_K1114208">E0030_CD</a>, and <a href="https://parts.igem.org/Part:BBA_K783020">B0015_DF</a> within the <a href="https://parts.igem.org/Part:BBa_K1114419">DVL1_EF</a> backbone. In the given sequence the internal fusion sites are incorrectly duplicated.
 
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.  
 
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.  
  
 
</html>
 
</html>
−
 
−
 
  
 
===Source===
 
===Source===

Latest revision as of 03:04, 13 September 2013


MoClo Level 1 Device that constitutively expresses YFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pJ04B2CY_EF This is a Level 1 MoClo device that constitutively expresses the reporter YFP. It contains the Level 0 parts J23104_EB, BCD2_BC, E0030_CD, and B0015_DF within the DVL1_EF backbone. In the given sequence the internal fusion sites are incorrectly duplicated. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.

Source

Construct of MoClo Level 0 parts derived from iGEM Distribution Kits

References