Difference between revisions of "Part:BBa K1114505"

Latest revision as of 02:13, 13 September 2013

MoClo Level 1 RFP reporter with AE fusion sites

pJ04B16Rm_AE This is a MoClo Level 1 reporting device containing the Level 0 parts J23104_AB, BCD16_BC, E1010m_CD, and B0015_DE within the DVL1_AE backbone. The flanking fusion sites are A on the 5' side and E on the 3' side of the insert. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. In the given sequence the internal fusion sites are incorrectly duplicated. See Level 0 pages for further information.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1238
Illegal XbaI site found at 1265
Illegal SpeI site found at 994
Illegal PstI site found at 1277
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1238
Illegal NheI site found at 11
Illegal NheI site found at 34
Illegal SpeI site found at 994
Illegal PstI site found at 1277
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1238
Illegal BamHI site found at 1259
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1238
Illegal XbaI site found at 1265
Illegal SpeI site found at 994
Illegal PstI site found at 1277
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1238
Illegal XbaI site found at 1265
Illegal SpeI site found at 994
Illegal PstI site found at 1277
Illegal AgeI site found at 710
Illegal AgeI site found at 822
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1502
Illegal BsaI.rc site found at 1014

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1114505 short</partinfo>
@@ Line 6: / Line 5: @@
 pJ04B16Rm_AE
-This is a <a href=""http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> Level 1 reporting device containing the Level 0 parts <a href="https://parts.igem.org/Part:BBa_K1114005">J23104_AB</a>, <a href="https://parts.igem.org/Part:BBa_K1114111:Design">BCD16_BC</a>, <a href="https://parts.igem.org/Part:BBa_K1114211">E1010m_CD</a>, and <a href="https://parts.igem.org/Part:BBa_K1114300">B0015_DE</a> within the <a href="https://parts.igem.org/Part:BBa_K1114418">DVL1_AE</a> backbone. The flanking fusion sites are A on the 5' side and E on the 3' side of the insert. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.
+This is a <a href=""http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> Level 1 reporting device containing the Level 0 parts <a href="https://parts.igem.org/Part:BBa_K1114005">J23104_AB</a>, <a href="https://parts.igem.org/Part:BBa_K1114111:Design">BCD16_BC</a>, <a href="https://parts.igem.org/Part:BBa_K1114211">E1010m_CD</a>, and <a href="https://parts.igem.org/Part:BBa_K1114300">B0015_DE</a> within the <a href="https://parts.igem.org/Part:BBa_K1114418">DVL1_AE</a> backbone. The flanking fusion sites are A on the 5' side and E on the 3' side of the insert. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. In the given sequence the internal fusion sites are incorrectly duplicated. See Level 0 pages for further information.
 </html>