Difference between revisions of "Part:BBa K1124000"
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| − | + | =amilGFP (+LVA)= | |
<b>yellow reporter protein with degradation tag.</b> | <b>yellow reporter protein with degradation tag.</b> | ||
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| − | amilGFP is a coral fluorescent protein from Acropora millepora. It is a member of the GFP family. | + | ==amilGFP== |
| + | is a coral fluorescent protein from Acropora millepora. It is a member of the GFP family. | ||
Excitation maximum : 503 nm | Excitation maximum : 503 nm | ||
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Molar Extinction : 75200 | Molar Extinction : 75200 | ||
---- | ---- | ||
| − | LVA tag is one of the ssrA degradation tag, which decreases the protein half-life when added to the C-terminus. | + | ==LVA tag== |
| + | is one of the ssrA degradation tag, which decreases the protein half-life when added to the C-terminus. | ||
---- | ---- | ||
| − | + | ==References== | |
Andersen, J. B., Sternberg, C., Poulsen, L. K., Bjørn, S. P., Givskov, M., & Molin, S. (1998). New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Applied and environmental microbiology, 64(6), 2240-2246. | Andersen, J. B., Sternberg, C., Poulsen, L. K., Bjørn, S. P., Givskov, M., & Molin, S. (1998). New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Applied and environmental microbiology, 64(6), 2240-2246. | ||
Labas, Y. A., Gurskaya, N. G., Yanushevich, Y. G., Fradkov, A. F., Lukyanov, K. A., Lukyanov, S. A., & Matz, M. V. (2002). Diversity and evolution of the green fluorescent protein family. Proceedings of the National Academy of Sciences, 99(7), 4256-4261. | Labas, Y. A., Gurskaya, N. G., Yanushevich, Y. G., Fradkov, A. F., Lukyanov, K. A., Lukyanov, S. A., & Matz, M. V. (2002). Diversity and evolution of the green fluorescent protein family. Proceedings of the National Academy of Sciences, 99(7), 4256-4261. | ||
Revision as of 02:23, 12 September 2013
amilGFP (+LVA)
yellow reporter protein with degradation tag.
The strong yellow color is visible to the naked eye under natural room light both in a LB liquid medium and on a LB agar plate. You can easily visualize gene expression without special equipment. Rapid degradation of the protein allows real-time imaging. It is also useful as a fluorescent reporter. For detailed usage, see UT-Tokyo 2013 wiki (currently under construction).
This is the LVA tagged version of BBa_K592010. LVA tag was added to increase decay rate by PCR using the primers shown on our wiki (UT-Tokyo 2013)(currently under construction).
amilGFP
is a coral fluorescent protein from Acropora millepora. It is a member of the GFP family.
Excitation maximum : 503 nm
Emission maximum : 512 nm
Quantum yield : 0.67
Molar Extinction : 75200
LVA tag
is one of the ssrA degradation tag, which decreases the protein half-life when added to the C-terminus.
References
Andersen, J. B., Sternberg, C., Poulsen, L. K., Bjørn, S. P., Givskov, M., & Molin, S. (1998). New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Applied and environmental microbiology, 64(6), 2240-2246.
Labas, Y. A., Gurskaya, N. G., Yanushevich, Y. G., Fradkov, A. F., Lukyanov, K. A., Lukyanov, S. A., & Matz, M. V. (2002). Diversity and evolution of the green fluorescent protein family. Proceedings of the National Academy of Sciences, 99(7), 4256-4261.