Difference between revisions of "Part:BBa K1124000"
YutaOtsuka (Talk | contribs) m (Created page with "amilGFP (+LVA) yellow reporter protein with degradation tag. The strong yellow color is visible to the naked eye under natural room light both in a LB liquid medium and on a LB ...") |
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| − | amilGFP (+LVA) | + | ===amilGFP (+LVA)=== |
| − | yellow reporter protein with degradation tag. | + | ===yellow reporter protein with degradation tag.=== |
The strong yellow color is visible to the naked eye under natural room light both in a LB liquid medium and on a LB agar plate. You can easily visualize gene expression without special equipment. Rapid degradation of the protein allows real-time imaging. It is also useful as a fluorescent reporter. For detailed usage, see UT-Tokyo 2013 wiki (currently under construction). | The strong yellow color is visible to the naked eye under natural room light both in a LB liquid medium and on a LB agar plate. You can easily visualize gene expression without special equipment. Rapid degradation of the protein allows real-time imaging. It is also useful as a fluorescent reporter. For detailed usage, see UT-Tokyo 2013 wiki (currently under construction). | ||
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This is the LVA tagged version of BBa_K592010. LVA tag was added to increase decay rate by PCR using the primers shown on our wiki (UT-Tokyo 2013)(currently under construction). | This is the LVA tagged version of BBa_K592010. LVA tag was added to increase decay rate by PCR using the primers shown on our wiki (UT-Tokyo 2013)(currently under construction). | ||
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amilGFP is a coral fluorescent protein from Acropora millepora. It is a member of the GFP family. | amilGFP is a coral fluorescent protein from Acropora millepora. It is a member of the GFP family. | ||
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Quantum yield : 0.67 | Quantum yield : 0.67 | ||
Molar Extinction : 75200 | Molar Extinction : 75200 | ||
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LVA tag is one of the ssrA degradation tag, which decreases the protein half-life when added to the C-terminus. | LVA tag is one of the ssrA degradation tag, which decreases the protein half-life when added to the C-terminus. | ||
| − | References | + | ===References=== |
Andersen, J. B., Sternberg, C., Poulsen, L. K., Bjørn, S. P., Givskov, M., & Molin, S. (1998). New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Applied and environmental microbiology, 64(6), 2240-2246. | Andersen, J. B., Sternberg, C., Poulsen, L. K., Bjørn, S. P., Givskov, M., & Molin, S. (1998). New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Applied and environmental microbiology, 64(6), 2240-2246. | ||
Labas, Y. A., Gurskaya, N. G., Yanushevich, Y. G., Fradkov, A. F., Lukyanov, K. A., Lukyanov, S. A., & Matz, M. V. (2002). Diversity and evolution of the green fluorescent protein family. Proceedings of the National Academy of Sciences, 99(7), 4256-4261. | Labas, Y. A., Gurskaya, N. G., Yanushevich, Y. G., Fradkov, A. F., Lukyanov, K. A., Lukyanov, S. A., & Matz, M. V. (2002). Diversity and evolution of the green fluorescent protein family. Proceedings of the National Academy of Sciences, 99(7), 4256-4261. | ||
Revision as of 02:18, 12 September 2013
amilGFP (+LVA)
yellow reporter protein with degradation tag.
The strong yellow color is visible to the naked eye under natural room light both in a LB liquid medium and on a LB agar plate. You can easily visualize gene expression without special equipment. Rapid degradation of the protein allows real-time imaging. It is also useful as a fluorescent reporter. For detailed usage, see UT-Tokyo 2013 wiki (currently under construction).
This is the LVA tagged version of BBa_K592010. LVA tag was added to increase decay rate by PCR using the primers shown on our wiki (UT-Tokyo 2013)(currently under construction).
amilGFP is a coral fluorescent protein from Acropora millepora. It is a member of the GFP family. Excitation maximum : 503 nm Emission maximum : 512 nm Quantum yield : 0.67 Molar Extinction : 75200
LVA tag is one of the ssrA degradation tag, which decreases the protein half-life when added to the C-terminus.
References
Andersen, J. B., Sternberg, C., Poulsen, L. K., Bjørn, S. P., Givskov, M., & Molin, S. (1998). New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Applied and environmental microbiology, 64(6), 2240-2246.
Labas, Y. A., Gurskaya, N. G., Yanushevich, Y. G., Fradkov, A. F., Lukyanov, K. A., Lukyanov, S. A., & Matz, M. V. (2002). Diversity and evolution of the green fluorescent protein family. Proceedings of the National Academy of Sciences, 99(7), 4256-4261.