Difference between revisions of "Part:BBa J23004"

 
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__NOTOC__
 
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<partinfo>BBa_J23004 short</partinfo>
 
<partinfo>BBa_J23004 short</partinfo>
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This plasmid is used to destroy the XbaI or SpeI restriction sites of a biobricked part.
 
This plasmid is used to destroy the XbaI or SpeI restriction sites of a biobricked part.
  
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Inserting an XbaI/PstI fragment of a biobrick part into the SpeI/PstI sites of pSB*dXS destroys the XbaI site resulting in a plasmid named pSB*dX-[part_name].
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Inserting an XbaI/PstI fragment of a biobrick part into the SpeI/PstI sites destroys the XbaI site
  
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Inserting an EcoRI/SpeI fragment of a biobrick part into the EcoRI/XbaI sites of pSB*dXS destroys the SpeI site resulting in a plasmid named pSB*dS-[part_name].
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Inserting an EcoRI/SpeI fragment of a biobrick part into the EcoRI/XbaI sites destroys the SpeI site  
  
 
To construct restiction-site deficient variants of a part in a different pSB* plasmid, transfer the EcoRI/PstI fragment into similar sites.
 
To construct restiction-site deficient variants of a part in a different pSB* plasmid, transfer the EcoRI/PstI fragment into similar sites.

Revision as of 02:06, 28 June 2006

Plasmid for destroying biobrick restriction sites

This plasmid is used to destroy the XbaI or SpeI restriction sites of a biobricked part.

Inserting an XbaI/PstI fragment of a biobrick part into the SpeI/PstI sites destroys the XbaI site

Inserting an EcoRI/SpeI fragment of a biobrick part into the EcoRI/XbaI sites destroys the SpeI site

To construct restiction-site deficient variants of a part in a different pSB* plasmid, transfer the EcoRI/PstI fragment into similar sites.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 957
    Illegal SpeI site found at 16
    Illegal PstI site found at 971
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 16
    Illegal PstI site found at 971
    Illegal NotI site found at 7
    Illegal NotI site found at 964
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 957
    Illegal SpeI site found at 16
    Illegal PstI site found at 971
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 957
    Illegal SpeI site found at 16
    Illegal PstI site found at 971
    Illegal AgeI site found at 667
    Illegal AgeI site found at 779
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2130