Difference between revisions of "Part:BBa J23005"
 
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<partinfo>BBa_J23005 short</partinfo>
 
<partinfo>BBa_J23005 short</partinfo>
  
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Biobricked cassette containing the chloramphenicol resistance gene flanked by FRT sites.  Useful for constructing markerless genome-integration cassettes.  The cassette is derived from pKD3 described by Datsenko&Wanner and can be excised by expression of flp recombinase in trans with helper plasmids such as pCP20.
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Biobricked cassette containing the chloramphenicol resistance gene flanked by FRT sites.  Useful for constructing markerless genome-integration cassettes.  The cassette is derived from pKD3 described by Datsenko & Wanner (PMID 10829079) and can be excised by expression of flp recombinase in trans with helper plasmids such as pCP20.
  
 
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Latest revision as of 13:27, 27 June 2006

[FRT-CmR-FRT]

Biobricked cassette containing the chloramphenicol resistance gene flanked by FRT sites. Useful for constructing markerless genome-integration cassettes. The cassette is derived from pKD3 described by Datsenko & Wanner (PMID 10829079) and can be excised by expression of flp recombinase in trans with helper plasmids such as pCP20.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 35
    Illegal XbaI site found at 965
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 35
    Illegal XbaI site found at 965
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 35
    Illegal XbaI site found at 965
  • 1000
    COMPATIBLE WITH RFC[1000]