Difference between revisions of "Part:BBa J45002:Design"

Revision as of 15:08, 26 June 2006

SAM:benzoic acid carboxyl methyltransferase; converts benzoic acid to methyl benzoate (floral odor)

Assembly Compatibility:

There are two Met codons at the N-terminus of the protein (putative codons 1 and 4). It is believed that the first Met codon is the actual start of the protein. Reference:

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Forward primer (5' -> 3'): GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATG AAA GTG ATG AAG AAA C
Reverse primer (5' -> 3'): GTT TCT TCC TGC AGC GGC CGC TAC TAG TA TTA TTA TCT CCT ACT TAG AGA AAC TAC

Bases 1-18 of the original A. majus sequence (gccggacgccaaagaaaa) were deleted. Thus, this sequence begins with the first start codon.
A silent mutation (A -> G) was made at base 702 to eliminate an SpeI cut site.
The original stop codon (TGA at bp 1093) was deleted and a double TAA stop was inserted

Genbank accession number(s): AF198492

We got this cDNA from N Dudareva at Purdue.

@@ Line 12: / Line 12: @@
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 #Forward primer (5' -> 3'): GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATG AAA GTG ATG AAG AAA C
+#Reverse primer (5' -> 3'): GTT TCT TCC TGC AGC GGC CGC TAC TAG TA TTA TTA TCT CCT ACT TAG AGA AAC TAC
 *Bases 1-18 of the original ''A. majus'' sequence (gccggacgccaaagaaaa) were deleted.  Thus, this sequence begins with the first start codon.
 *A silent mutation (A -> G) was made at base 702 to eliminate an SpeI cut site.