Difference between revisions of "Part:BBa K1123011:Design"
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No considerations were made other than ensuring that no hair pins or dimers could be formed with the designed spacer. We also ensured no illegal restriction sites occurred. | No considerations were made other than ensuring that no hair pins or dimers could be formed with the designed spacer. We also ensured no illegal restriction sites occurred. | ||
We did however add a stop codon and a XhoI restriction site to the very start of this sequence. The stop codon would allow us to stop protein expression wand the restriction site would allow us to restrict the protein sequence from the larger construct for transferal into a different vector. | We did however add a stop codon and a XhoI restriction site to the very start of this sequence. The stop codon would allow us to stop protein expression wand the restriction site would allow us to restrict the protein sequence from the larger construct for transferal into a different vector. | ||
| + | The Stop codon are the first 3 base pairs and the restriction site is formed by the next 6 base pairs. | ||
===Source=== | ===Source=== | ||
Latest revision as of 12:16, 8 September 2013
Spacer Sequence
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 4
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
No considerations were made other than ensuring that no hair pins or dimers could be formed with the designed spacer. We also ensured no illegal restriction sites occurred. We did however add a stop codon and a XhoI restriction site to the very start of this sequence. The stop codon would allow us to stop protein expression wand the restriction site would allow us to restrict the protein sequence from the larger construct for transferal into a different vector. The Stop codon are the first 3 base pairs and the restriction site is formed by the next 6 base pairs.
Source
This part was designed by ourselves. It was constructed by using a randomising a DNA sequence.