Difference between revisions of "Part:BBa J45001:Design"

(→‎Design Notes)
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#primer design
 
#primer design
 
#*forward = 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G<b>AT GAC AAA ACA AAC ACA AAA G</b> -3'
 
#*forward = 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G<b>AT GAC AAA ACA AAC ACA AAA G</b> -3'
−
#*reverse = 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TA<b>ctctcgccttgtcatggaaatag</b> -3'
+
#*reverse = 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TA<b>c tct cgc ctt gtc atg gaa ata g</b> -3'
  
 
===Source===
 
===Source===

Revision as of 14:56, 26 June 2006


SAM:salicylic acid carboxyl methyltransferase; converts salicylic acid to methyl salicylate (winter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 577
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 901
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 421
    Illegal NgoMIV site found at 425
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 471
    Illegal SapI.rc site found at 75


Design Notes

  1. no restriction enzyme cut sites in coding region
  2. changed natural stop codon to TAA TAA
  3. primer design
    • forward = 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAT GAC AAA ACA AAC ACA AAA G -3'
    • reverse = 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAc tct cgc ctt gtc atg gaa ata g -3'

Source

  1. GenBank Accession No. AF515284
  2. Species = Antirrhinum Majus, Snapdragon

References

  1. corresponding author: Natalia Dudareva
  2. Pubmed: PMID: 12361714
  3. Abstract for SAMT enzyme:
    1. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12361714&dopt=Abstract
  4. Wiki Link:
    1. http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-6-6