Difference between revisions of "Featured Parts:Conjugation parts"

(→‎Part Type: Conjugation Parts)
(→‎Part Type: Conjugation Parts)
Line 5: Line 5:
 
<small> Bacterial conjugation is the transfer of genetic material between cells.  This behaviour is characterized by the presence of a specialized plasmid (small, circular piece of transferrable DNA most often found within bacteria) known as a '' "conjugative plasmid" ''.  <br> The conjugative plasmid holds on it a distinct and specialized set of coding regions including the following
 
<small> Bacterial conjugation is the transfer of genetic material between cells.  This behaviour is characterized by the presence of a specialized plasmid (small, circular piece of transferrable DNA most often found within bacteria) known as a '' "conjugative plasmid" ''.  <br> The conjugative plasmid holds on it a distinct and specialized set of coding regions including the following
 
* OriT (Origin of Transfer):  Unlike other plasmids, the conjugative plasmid has its own origin of transfer.   
 
* OriT (Origin of Transfer):  Unlike other plasmids, the conjugative plasmid has its own origin of transfer.   
−
* TraJ (Transfer genes):  The activation of this set of genes on the conjugative plasmid sets off a cascade of protein activation that forces the host cell to start expressing surface proteins that form "mating" characteristics.  Such salient characteristics include:
+
* TraJ (Transfer genes):  The activation of this set of genes on the conjugative plasmid sets off a cascade that forces the host cell to start expressing surface proteins that form "mating" characteristics.  Such salient characteristics include:
 
** the growth of a pilus (a long whip-like apparatus used for genetic transfer between cells)
 
** the growth of a pilus (a long whip-like apparatus used for genetic transfer between cells)
−
** formation of "surface exclusion proteins" which prevents the cell containing the conjugative plasmid from mating with other conjugative plasmids of its type (ie. F-type plasmid bearers manufacture surface exclusion proteins that prevent it from mating with other F-type cells although it will mate with cells bearing the R-type plasmid)
+
** formation of "surface exclusion proteins" which prevents the cell containing the conjugative plasmid from mating with other conjugative plasmids of its type  
  
 
References:<br>
 
References:<br>
 
1.  Frost, Ipp en-Ihler, Skurray.  Analysis and Sequence of the F Plasmid  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7915817&query_hl=2&itool=pubmed_docsum Pubmed]<br>
 
1.  Frost, Ipp en-Ihler, Skurray.  Analysis and Sequence of the F Plasmid  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7915817&query_hl=2&itool=pubmed_docsum Pubmed]<br>
−
2.  Wikipedia.  "Bacterial Conjugation" [http://en.wikipedia.org/wiki/Bacterial_conjugation Link]
+
2.  Wikipedia.  "Bacterial Conjugation" [http://en.wikipedia.org/wiki/Bacterial_conjugation Link] <br>
 +
3.  Berkeley iGEM 2005 Project
 
</small>
 
</small>
−
3.  Berkeley iGEM 2005 Project [[Berkeley]]
 

Revision as of 02:19, 30 March 2006

Part Type: Conjugation Parts

About Conjugation: Bacterial conjugation is the transfer of genetic material between cells. This behaviour is characterized by the presence of a specialized plasmid (small, circular piece of transferrable DNA most often found within bacteria) known as a "conjugative plasmid" .
The conjugative plasmid holds on it a distinct and specialized set of coding regions including the following

  • OriT (Origin of Transfer): Unlike other plasmids, the conjugative plasmid has its own origin of transfer.
  • TraJ (Transfer genes): The activation of this set of genes on the conjugative plasmid sets off a cascade that forces the host cell to start expressing surface proteins that form "mating" characteristics. Such salient characteristics include:
    • the growth of a pilus (a long whip-like apparatus used for genetic transfer between cells)
    • formation of "surface exclusion proteins" which prevents the cell containing the conjugative plasmid from mating with other conjugative plasmids of its type

References:
1. Frost, Ipp en-Ihler, Skurray. Analysis and Sequence of the F Plasmid [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7915817&query_hl=2&itool=pubmed_docsum Pubmed]
2. Wikipedia. "Bacterial Conjugation" [http://en.wikipedia.org/wiki/Bacterial_conjugation Link]
3. Berkeley iGEM 2005 Project