Difference between revisions of "Part:BBa K1114101:Design"

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This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' end of sequence.
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This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' end of sequence.
 
The fusion site letters refer to 4bp fusion sites: A        = GGAG;        B        = TACT; C        = AATG; D        = AGGT;        E        = GCTT; F        = CGCT; G        = TGCC; H        = ACTA.
 
The fusion site letters refer to 4bp fusion sites: A        = GGAG;        B        = TACT; C        = AATG; D        = AGGT;        E        = GCTT; F        = CGCT; G        = TGCC; H        = ACTA.
  
 
Summary of modifications from original part:
 
Summary of modifications from original part:
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Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
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Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.  See <a href=”https://parts.igem.org/Part:BBa_K783055“>BBa_K783055</a>.
  
 
===Source===
 
===Source===

Revision as of 18:40, 7 September 2013

MoClo format of a modified version of BBa_B0031 with BC fusion sites.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is the MoClo formatted and modified part of BBa_B0031 with BC fusion sites. See the BBa_B0031 page for full information on the part.

This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' end of sequence. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

Summary of modifications from original part:

Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href=”https://parts.igem.org/Part:BBa_K783055“>BBa_K783055</a>.

Source

iGEM Distribution Kit

References