Difference between revisions of "Part:BBa K1114202"
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<partinfo>BBa_K1114202 short</partinfo> | <partinfo>BBa_K1114202 short</partinfo> | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
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+ | ===Applications of BBa_K1114201=== | ||
+ | <html> | ||
+ | This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_C0040 with CD fusion sites. See the <a href="https://parts.igem.org/Part:BBa_C0040">BBa_C0040</a> page for full information on the part. | ||
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+ | This is a Level 0 MoClo part with flanking sites C on the 5' side and site D on the 3' side of the part. | ||
+ | The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. | ||
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+ | Summary of modifications from original part: | ||
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+ | Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href="https://parts.igem.org/Part:pSB1C3?title=Part:pSB1C3">pSB1C3</a>. | ||
+ | </html> | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 18:31, 7 September 2013
C0040_CD, Moclo of BBA_C0040
Moclo analogue of bba_c0040 with C and D fusion sites added
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]