Difference between revisions of "Part:BBa K1114202"

 
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<partinfo>BBa_K1114202 short</partinfo>
 
<partinfo>BBa_K1114202 short</partinfo>
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===Applications of BBa_K1114201===
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This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_C0040 with CD fusion sites. See the <a href="https://parts.igem.org/Part:BBa_C0040">BBa_C0040</a> page for full information on the part.
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This is a Level 0 MoClo part with flanking sites C on the 5' side and site D on the 3' side of the part.
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The fusion site letters refer to 4bp fusion sites: A        = GGAG;        B        = TACT; C        = AATG; D        = AGGT;        E        = GCTT; F        = CGCT; G        = TGCC; H        = ACTA.
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Summary of modifications from original part:
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Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.  See <a href="https://parts.igem.org/Part:pSB1C3?title=Part:pSB1C3">pSB1C3</a>.
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===Usage and Biology===
 
===Usage and Biology===
  

Revision as of 18:31, 7 September 2013

C0040_CD, Moclo of BBA_C0040

Moclo analogue of bba_c0040 with C and D fusion sites added

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]