Difference between revisions of "Part:BBa K1114001:Design"

 
(→‎Design Notes)
Line 4: Line 4:
  
 
<partinfo>BBa_K1114001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1114001 SequenceAndFeatures</partinfo>
 +
 +
 +
 +
 +
  
  
Line 9: Line 14:
 
<html>
 
<html>
 
This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_J23101 with AB fusion sites. See the <a href="https://parts.igem.org/Part:BBa_J23101">BBa_J23101</a> page for full information on the part.  
 
This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_J23101 with AB fusion sites. See the <a href="https://parts.igem.org/Part:BBa_J23101">BBa_J23101</a> page for full information on the part.  
 +
 +
 +
 +
This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part.
 +
The fusion site letters refer to 4bp fusion sites: A        = GGAG;        B        = TACT; C        = AATG; D        = AGGT;        E        = GCTT; F        = CGCT; G        = TGCC; H        = ACTA.
 +
 +
 +
Summary of modifications from original part:
 +
 +
 +
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.  See <a href=”https://parts.igem.org/Part:BBa_K783055“>BBa_K783055 </a>.
 +
 +
 
</html>
 
</html>
  
−
Summary of Modifications from original part:
+
 
−
Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
+
===Source===
  
  
 +
iGEM Distribution Kit
  
  
 +
===References===
  
 
===Source===
 
===Source===

Revision as of 18:29, 7 September 2013


The MoClo format of BBa_J23101 with AB fusion sites.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]




Design Notes

This is the MoClo formatted part of BBa_J23101 with AB fusion sites. See the BBa_J23101 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 .


Source

iGEM Distribution Kit


References

Source

iGEM Distribution Kit

References