Difference between revisions of "Part:BBa K1114033:Design"

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<a href="http://2013.igem.org/Team:Purdue">Purdue Biomaker's iGEM Team</a>
 
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===Design Notes===
 
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In a MoClo backbone, <a href="https://www.google.com/">BBa_DVL0_FB</a>.
 
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<a href="http://2013.igem.org/Team:Purdue">Purdue Biomaker's iGEM Team</a>
 
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Revision as of 18:15, 7 September 2013

MoClo formatted part of BBa_R0079 with FB fusion sites


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 62
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is the MoClo formatted part of BBa_R0079 with FB fusion sites. See the BBa_R0079 page for full information on the part.

This is a Level 0 MoClo part with flanking sites F on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.

Source

iGEM Kit

References