Difference between revisions of "Part:BBa K1114034:Design"

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<partinfo>BBa_K1114034 short</partinfo>
 
<partinfo>BBa_K1114034 short</partinfo>

Revision as of 17:43, 7 September 2013

The MoClo format of BBa_R0010 with FB fusion sites


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is the MoClo formatted part of BBa_R0010 with FB fusion sites. See the BBa_R0010 page for full information on the part.

This is a Level 0 MoClo destination vector with flanking sites A on the 5' side and site B on the 3' side of the alpha fragment of lacZ. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.

Source

iGEM Kit

References