Difference between revisions of "Part:BBa K1114005:Design"

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===Design Notes===
 
===Design Notes===
 
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This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_J23104 with EB fusion sites. See the <a href="https://parts.igem.org/Part:BBa_R0079">BBa_J23104</a> page for full information on the part.  
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This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_J23104 with AB fusion sites. See the <a href="https://parts.igem.org/Part:BBa_R0079">BBa_J23104</a> page for full information on the part.  
 
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Latest revision as of 17:20, 7 September 2013


The MoClo format of BBa_J23104 with AB fusion sites.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is the MoClo formatted part of BBa_J23104 with AB fusion sites. See the BBa_J23104 page for full information on the part.

Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.

The fusion site letters refer to 4bp fusion sites: A=GGAG; B=TACT; C=AATG; D=AGGT; E=GCTT; F = CGCT; G=TGCC; H=ACTA.

Source

iGEM Distribution Kit

References