Difference between revisions of "Part:BBa K1138001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | Primers were designed in a way to introduce EcoRI-NotI-XbaI-NsiI sites before the linker. | |
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===Source=== | ===Source=== | ||
Latest revision as of 16:48, 6 September 2013
pAW50-linker-mcherry(-RBS) (ugaiGEM11)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 199
Illegal EcoRI site found at 205
Illegal XbaI site found at 220
Illegal SpeI site found at 969
Illegal PstI site found at 609
Illegal PstI site found at 976 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 199
Illegal EcoRI site found at 205
Illegal SpeI site found at 969
Illegal PstI site found at 609
Illegal PstI site found at 976
Illegal NotI site found at 211 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 199
Illegal EcoRI site found at 205 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 205
Plasmid lacks a suffix.
Illegal EcoRI site found at 199
Illegal SpeI site found at 969
Illegal PstI site found at 609
Illegal PstI site found at 976 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 205
Plasmid lacks a suffix.
Illegal EcoRI site found at 199
Illegal XbaI site found at 220
Illegal SpeI site found at 969
Illegal PstI site found at 609
Illegal PstI site found at 976
Illegal AgeI site found at 987 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 3145
Illegal BsaI.rc site found at 1528
Illegal BsaI.rc site found at 1857
Illegal SapI site found at 4809
Design Notes
Primers were designed in a way to introduce EcoRI-NotI-XbaI-NsiI sites before the linker.
Source
mcherry was obtained from Discosoma sp.