Difference between revisions of "Part:BBa K1216006"
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Acetyl esterase - This is a cytosolic hydrolase that can catalyze hydrlysis of esters of p-nitrophenyl derivatives  
 
Acetyl esterase - This is a cytosolic hydrolase that can catalyze hydrlysis of esters of p-nitrophenyl derivatives  
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[[File:Acetyl_esterase_3D.jpg|thumb| 3D representation of the acetyl esterase from [http://www.rcsb.org/pdb/explore/explore.do?structureId=4KRX RCSB] ]]  
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[[File:Acetyl_esterase_3D.jpg|thumb| 3D representation of the acetyl esterase from [http://www.rcsb.org/pdb/explore/explore.do?structureId=4KRX RCSB] ]] The poly-HIS tag can be used for protein purification (IMAC)[[Part:BBa_K1216001#References|<sup>[1]</sup>]]. The TEV tag can then be used to have the TEV protease specifically cleave off the poly-HIS tag from the purified protein [[Part:BBa_K1216001#References|<sup>[2]</sup>]].
  
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A form of this protein with added TEV and poly-HIS tags can be found [[Part:BBa_K1216006| here]].
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A form of this protein without TEV and poly-HIS tags can be found [[Part:BBa_K1216002| here]].
 
<!-- Add more about the biology of this part here -->
 
<!-- Add more about the biology of this part here -->
 
===Usage and Biology===
 
===Usage and Biology===
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The hydrolase capacity of acetyl esterase makes it suitable for reporter application with substrates like acetylated xylan, ethyl acetate, cephalosporin C and derivatives[[Part:BBa_K1216002#References|<sup>[1]</sup>]] . Further it can be used for desacteylation of β-Lactam antibiotics[[Part:BBa_K1216002#References|<sup>[1]</sup>]] .
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The hydrolase capacity of acetyl esterase makes it suitable for reporter application with substrates like acetylated xylan, ethyl acetate, cephalosporin C and derivatives[[Part:BBa_K1216002#References|<sup>[3]</sup>]] . Further it can be used for desacteylation of β-Lactam antibiotics[[Part:BBa_K1216002#References|<sup>[3]</sup>]] .
  
  
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The acetyle esterase, apart from its hydrolase activity also binds competitively to malT, a transcription activator for the maltose operon, thus inhibiting regulation of genes required for maltose catabolism[[Part:BBa_K1216002#References|<sup>[2]</sup>]] [[Part:BBa_K1216002#References|<sup>[3]</sup>]].
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The acetyle esterase, apart from its hydrolase activity also binds competitively to malT, a transcription activator for the maltose operon, thus inhibiting regulation of genes required for maltose catabolism[[Part:BBa_K1216002#References|<sup>[4]</sup>]] [[Part:BBa_K1216002#References|<sup>[5]</sup>]].
  
  
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===References===
 
===References===
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# Loghran ST, "Purification of poly-histidine-tagged proteins.",Methods Mol Biol. 2011;681:311-35. doi: 10.1007/978-1-60761-913-0_17.[http://www.ncbi.nlm.nih.gov/pubmed/20978973]
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# [http://homepage.univie.ac.at/nikos.pinotsis/tev_protease.html University of Vienna TEV Protease info]
 
# [http://www.cpcbiotech.it/EN/c/d/enzyme-portfolio/enzymes/acetyl-esterase-lyophilized CPC Biotech]
 
# [http://www.cpcbiotech.it/EN/c/d/enzyme-portfolio/enzymes/acetyl-esterase-lyophilized CPC Biotech]
 
# [http://www.ecocyc.org/new-image?type=GENE&object=EG11101 Ecocyc]
 
# [http://www.ecocyc.org/new-image?type=GENE&object=EG11101 Ecocyc]
 
# [http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=MONOMER0-158&orgids=ECOLI Ecocyc]
 
# [http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=MONOMER0-158&orgids=ECOLI Ecocyc]

Revision as of 13:11, 6 September 2013

Acetyl esterase (aes) from Escherichia Coli with TEV and poly-HIS tags

Acetyl esterase - This is a cytosolic hydrolase that can catalyze hydrlysis of esters of p-nitrophenyl derivatives

3D representation of the acetyl esterase from [http://www.rcsb.org/pdb/explore/explore.do?structureId=4KRX RCSB]
The poly-HIS tag can be used for protein purification (IMAC)[1]. The TEV tag can then be used to have the TEV protease specifically cleave off the poly-HIS tag from the purified protein [2].

A form of this protein without TEV and poly-HIS tags can be found here.

Usage and Biology

The hydrolase capacity of acetyl esterase makes it suitable for reporter application with substrates like acetylated xylan, ethyl acetate, cephalosporin C and derivatives[3] . Further it can be used for desacteylation of β-Lactam antibiotics[3] .


The acetyle esterase, apart from its hydrolase activity also binds competitively to malT, a transcription activator for the maltose operon, thus inhibiting regulation of genes required for maltose catabolism[4] [5].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 27
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 951


References

  1. Loghran ST, "Purification of poly-histidine-tagged proteins.",Methods Mol Biol. 2011;681:311-35. doi: 10.1007/978-1-60761-913-0_17.[http://www.ncbi.nlm.nih.gov/pubmed/20978973]
  2. [http://homepage.univie.ac.at/nikos.pinotsis/tev_protease.html University of Vienna TEV Protease info]
  3. [http://www.cpcbiotech.it/EN/c/d/enzyme-portfolio/enzymes/acetyl-esterase-lyophilized CPC Biotech]
  4. [http://www.ecocyc.org/new-image?type=GENE&object=EG11101 Ecocyc]
  5. [http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=MONOMER0-158&orgids=ECOLI Ecocyc]