Difference between revisions of "Part:BBa K1216006"

 
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__NOTOC__
 
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<partinfo>BBa_K1216006 short</partinfo>
 
<partinfo>BBa_K1216006 short</partinfo>
  
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acetyl esterase with a His tag
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Acetyl esterase - This is a cytosolic hydrolase that can catalyze hydrlysis of esters of p-nitrophenyl derivatives
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[[File:Acetyl_esterase_3D.jpg|thumb| 3D representation of the acetyl esterase from [http://www.rcsb.org/pdb/explore/explore.do?structureId=4KRX RCSB] ]]
  
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<!-- Add more about the biology of this part here
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A form of this protein with added TEV and poly-HIS tags can be found [[Part:BBa_K1216006| here]].
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<!-- Add more about the biology of this part here -->
 
===Usage and Biology===
 
===Usage and Biology===
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The hydrolase capacity of acetyl esterase makes it suitable for reporter application with substrates like acetylated xylan, ethyl acetate, cephalosporin C and derivatives[[Part:BBa_K1216002#References|<sup>[1]</sup>]] . Further it can be used for desacteylation of β-Lactam antibiotics[[Part:BBa_K1216002#References|<sup>[1]</sup>]] .
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The acetyle esterase, apart from its hydrolase activity also binds competitively to malT, a transcription activator for the maltose operon, thus inhibiting regulation of genes required for maltose catabolism[[Part:BBa_K1216002#References|<sup>[2]</sup>]] [[Part:BBa_K1216002#References|<sup>[3]</sup>]].
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1216006 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1216002 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K1216006 parameters</partinfo>
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<partinfo>BBa_K1216002 parameters</partinfo>
 
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===References===
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# [http://www.cpcbiotech.it/EN/c/d/enzyme-portfolio/enzymes/acetyl-esterase-lyophilized CPC Biotech]
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# [http://www.ecocyc.org/new-image?type=GENE&object=EG11101 Ecocyc]
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# [http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=MONOMER0-158&orgids=ECOLI Ecocyc]

Revision as of 13:09, 6 September 2013

Acetyl esterase (aes) from Escherichia Coli with TEV and poly-HIS tags

Acetyl esterase - This is a cytosolic hydrolase that can catalyze hydrlysis of esters of p-nitrophenyl derivatives

3D representation of the acetyl esterase from [http://www.rcsb.org/pdb/explore/explore.do?structureId=4KRX RCSB]

A form of this protein with added TEV and poly-HIS tags can be found here.

Usage and Biology

The hydrolase capacity of acetyl esterase makes it suitable for reporter application with substrates like acetylated xylan, ethyl acetate, cephalosporin C and derivatives[1] . Further it can be used for desacteylation of β-Lactam antibiotics[1] .


The acetyle esterase, apart from its hydrolase activity also binds competitively to malT, a transcription activator for the maltose operon, thus inhibiting regulation of genes required for maltose catabolism[2] [3].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 27
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 951


References

  1. [http://www.cpcbiotech.it/EN/c/d/enzyme-portfolio/enzymes/acetyl-esterase-lyophilized CPC Biotech]
  2. [http://www.ecocyc.org/new-image?type=GENE&object=EG11101 Ecocyc]
  3. [http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=MONOMER0-158&orgids=ECOLI Ecocyc]