Difference between revisions of "Part:BBa K1216002"
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Acetyl esterase - This is a cytosolic hydrolase that can catalyze hydrlysis of esters of p-nitrophenyl derivatives | Acetyl esterase - This is a cytosolic hydrolase that can catalyze hydrlysis of esters of p-nitrophenyl derivatives | ||
| + | [[File:Acetyl_esterase_3D.jpg|thumb| 3D representation of the acetyl esterase from [http://www.rcsb.org/pdb/explore/explore.do?structureId=4KRX RCSB] ]] | ||
A form of this protein with added TEV and poly-HIS tags can be found [[Part:BBa_K1216006| here]]. | A form of this protein with added TEV and poly-HIS tags can be found [[Part:BBa_K1216006| here]]. | ||
Revision as of 09:49, 6 September 2013
Acetyl esterase (aes) from Escherichia Coli
Acetyl esterase - This is a cytosolic hydrolase that can catalyze hydrlysis of esters of p-nitrophenyl derivatives
A form of this protein with added TEV and poly-HIS tags can be found here.
Usage and Biology
The hydrolase capacity of acetyl esterase makes it suitable for reporter application with substrates like acetylated xylan, ethyl acetate, cephalosporin C and derivatives[1] . Further it can be used for desacteylation of β-Lactam antibiotics[1] .
The acetyle esterase, apart from its hydrolase activity also binds competitively to malT, a transcription activator for the maltose operon, thus inhibiting regulation of genes required for maltose catabolism[2] [3].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 27
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 951
References
- [http://www.cpcbiotech.it/EN/c/d/enzyme-portfolio/enzymes/acetyl-esterase-lyophilized CPC Biotech]
- [http://www.ecocyc.org/new-image?type=GENE&object=EG11101 Ecocyc]
- [http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=MONOMER0-158&orgids=ECOLI Ecocyc]