Difference between revisions of "Part:BBa K1216000"

Revision as of 08:05, 6 September 2013

β-Glucuronidase (gusA) from Bacillis Subtilis

gusA (also called uidA^[1]) encodes β-Glucuronidase, an intracellular enzyme that catalyzes the hydrolysis of β-D-glucuronides.

A form of this protein with added TEV and poly-HIS tags can be found here.

Add more about the biology of this part here

Usage and Biology

β-Glucuronidase is used as a fusion protein marker in higher plants, due to them lacking intrinsic β-Glucuronidase activity^[2]. Generally it can be used as reporter enzyme with detection by biochemical activity assays, immunological assays or by histochemical staining of tissue sections or cells^[3]. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 538
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

[http://ecoliwiki.net/colipedia/index.php/uidA:Gene ecoliwiki]
Jefferson A R, "GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.", EMBO J. 1987 December 20; 6(13): 3901–3907 [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC553867/]

@@ Line 6: / Line 6: @@
 A form of this protein with added TEV and poly-HIS tags can be found [[Part:BBa_K1216005| here]].
+Add more about the biology of this part here
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
 β-Glucuronidase is used as a fusion protein marker in higher plants, due to them lacking intrinsic β-Glucuronidase activity[[Part:BBa_K1216000#References|<sup>[2]</sup>]].
+Generally it can be used as reporter enzyme with detection by biochemical activity assays, immunological assays or by histochemical staining of tissue sections or cells[[Part:BBa_K1216000#References|<sup>[3]</sup>]].
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>