Difference between revisions of "Part:BBa K1036000"
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=Experimental Data= | =Experimental Data= | ||
Revision as of 09:12, 3 September 2013
lux pL controlled luxR with lux pR controlled ndh (LVA-tag) coding for NADH dehydrogenase II
The ndh gene codes for NDH-2, an enzyme that generates
Usage and Biology
Protocol
Ndh with LVA tag is formed as a composite by the ligation of quorum sensing promoter luxR via digestion and ligation.
Experimental Data
SDS page, microfluid, and gel image of construction.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2544
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1101