Difference between revisions of "Part:BBa K1216001"
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===Usage and Biology===
 
===Usage and Biology===
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Alkaline phosphatases are used as reporter enzymes in different assays such as Western Blotting and in situ hybridization. [[Part:BBa_K1216001#References|[1]]]
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Alkaline phosphatases are used as reporter enzymes in different assays such as Western Blotting and in situ hybridization. [[Part:BBa_K1216001#References|<sup>[1]</sup>]]
  
  

Revision as of 05:52, 2 September 2013

Alkaline Phosphatase (phoA) from Citrobacter

The alkaline phosphatase is a periplasmic homodimeric hydrolase.

Usage and Biology

Alkaline phosphatases are used as reporter enzymes in different assays such as Western Blotting and in situ hybridization. [1]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 340
    Illegal NgoMIV site found at 787
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Molecular Cell Biology, Fifth Edition, W.H. Freeman & Co., 2004.