Difference between revisions of "Part:BBa K1123014:Design"

 
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<partinfo>BBa_K1123002 short</partinfo>
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<partinfo>BBa_K1123014 short</partinfo>
  
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<partinfo>BBa_K1123002 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1123014 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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The protein sequence was codon optimized for expression in E.coli bacteria.  
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The protein sequence was codon optimized for expression in E.coli bacteria. We then repeated the sequence a number of times to amplify the protein. These repeats were held together using small linkage sequences of Glycine and Serine amino acids. In total the 1ETF protein sequence was repeated 5 times.
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===Source===
 
===Source===

Latest revision as of 12:08, 31 August 2013


DNA Coding Sequence for 1ETF Protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The protein sequence was codon optimized for expression in E.coli bacteria. We then repeated the sequence a number of times to amplify the protein. These repeats were held together using small linkage sequences of Glycine and Serine amino acids. In total the 1ETF protein sequence was repeated 5 times.

Source

The amino acid sequence for this parts was retrieved from PDB.org after a database query was performed looking for proteins with high Arginine and Lysine contents.

References