Difference between revisions of "Part:BBa K1123000:Design"

 
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===Design Notes===
 
===Design Notes===
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To remove the EcoRI site we adapted the tail of the sequence which we had found in the above mentioned article. The EcoRI site was situated in at -132. We therefore removed the sequence from -132 to -116 and replaced it with a sequence of 4 base pairs namely: GGTA. We hoped that this adaptation would be of no influence on the efficiency of the promoter.  
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To remove the EcoRI site we adapted the tail of the sequence which we had found in the below mentioned article. The EcoRI site was situated in at -132. We therefore removed the sequence from -132 to -116 and replaced it with a sequence of 4 base pairs namely: GGTA. We hoped that this adaptation would be of no influence on the efficiency of the promoter.
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===Source===
 
===Source===

Latest revision as of 11:45, 31 August 2013


FNR Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 39
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To remove the EcoRI site we adapted the tail of the sequence which we had found in the below mentioned article. The EcoRI site was situated in at -132. We therefore removed the sequence from -132 to -116 and replaced it with a sequence of 4 base pairs namely: GGTA. We hoped that this adaptation would be of no influence on the efficiency of the promoter.

Source

Part sourced from the following study into the FNR promomoter: "Transcriptional Regulation by Tandem-Bound FNR at Escherichia coli Promoters", Barnard et al.

References