Difference between revisions of "Part:BBa K1218014:Design"

 
(→‎References)
 
Line 16: Line 16:
  
 
===References===
 
===References===
 +
[[http://nar.oxfordjournals.org/content/41/15/7429]] David Bikard, Wenyan Jiang, Poulami Samai, Ann Hochschild, Feng Zhang3, and Luciano A. Marraffini. (2013) Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. ''Nucleic Acids Research'', 41 (15), 7429-7437.

Latest revision as of 01:56, 30 August 2013


dCas9-ω Activator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1642
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3921
    Illegal BamHI site found at 4755
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mutagenesis to remove internal EcoRI site


Source

CRISPR-Cas system from streptococcus pyogenes; cloned from plasmids obtained from Bikard et al.

References

http://nar.oxfordjournals.org/content/41/15/7429 David Bikard, Wenyan Jiang, Poulami Samai, Ann Hochschild, Feng Zhang3, and Luciano A. Marraffini. (2013) Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic Acids Research, 41 (15), 7429-7437.