Difference between revisions of "Part:BBa K1218011:Design"

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===References===
 
===References===
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[[http://nar.oxfordjournals.org/content/41/15/7429]]
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[[http://nar.oxfordjournals.org/content/41/15/7429]] David Bikard, Wenyan Jiang, Poulami Samai, Ann Hochschild, Feng Zhang3, and Luciano A. Marraffini. (2013) Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. ''Nucleic Acids Research'', 41 (15), 7429-7437.

Revision as of 01:42, 30 August 2013


Cas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1642
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3921
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4863
    Illegal BsaI.rc site found at 4840


Design Notes

Mutagenesis to remove internal EcoRI site


Source

CRISPR-Cas system from streptococcus pyogenes; cloned from plasmids obtained from Bikard et al.

References

http://nar.oxfordjournals.org/content/41/15/7429 David Bikard, Wenyan Jiang, Poulami Samai, Ann Hochschild, Feng Zhang3, and Luciano A. Marraffini. (2013) Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic Acids Research, 41 (15), 7429-7437.