Difference between revisions of "Part:BBa K1185001:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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There are no illegal restriction sites in this BioBrick. As the ''hbs'' gene is found naturally in ''B. subtilis'' there is no need for codon optimization. We chose the linker sequence as it is flexible and so does not interfere with the function of the fusion protein. We have included a three frame stop codon at the end of our construct.
+
There are no illegal restriction sites in this BioBrick. As the ''hbs'' gene is found naturally in ''B. subtilis'' there is no need for codon optimization. We chose the linker sequence as it is flexible and so does not interfere with the function of the fusion protein. The sfGFP BioBrick has already undergone codon optimization for ''B. subtilis''. We have included a three frame stop codon at the end of our construct.
  
 
===Source===
 
===Source===

Revision as of 12:01, 29 August 2013


HBsu-sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 339


Design Notes

There are no illegal restriction sites in this BioBrick. As the hbs gene is found naturally in B. subtilis there is no need for codon optimization. We chose the linker sequence as it is flexible and so does not interfere with the function of the fusion protein. The sfGFP BioBrick has already undergone codon optimization for B. subtilis. We have included a three frame stop codon at the end of our construct.

Source

The source of the HBsu coding sequence was the B. subtilis strain 168 genome, SwissProtTM accession number: P08821. The sfGFP sequence was sourced from BBa_I746916 and the linker from: BBa_K105012.

References