Difference between revisions of "Part:BBa K1041000"
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Image:J04450 - lightbox.jpg|BBa_J04450 visualized under non-UV lightbox
 
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Image:J04450 - UV 254nm.jpg|BBa_J04450 visualized under 254nm wavelength UV lightbox
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Image:J04500-colonies.jpg|BBa_J04450 Colonies
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Revision as of 11:06, 24 August 2013

RFP Coding Device

Mutagenesis was used to add a Nde1 site at the start of the RFP coding region of the biobrick BBa_J04450. This enables a restriction digest with Nde1 to be performed allowing the RFP coding region to be excised from the plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]

Pictures

  • BBa_J04450 visualized under non-UV lightbox

  • ===Functional Parameters===