Difference between revisions of "Part:BBa K1051702:Design"
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===Design Notes=== | ===Design Notes=== | ||
Assembly standard 23 | Assembly standard 23 | ||
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| + | The optimal PCR temperature should be assessed by gradient PCR first in order to maximize the PCR product | ||
===Source=== | ===Source=== | ||
Latest revision as of 12:06, 23 August 2013
SRC1 Intron+CGG
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Assembly standard 23
The optimal PCR temperature should be assessed by gradient PCR first in order to maximize the PCR product
Source
From SRC1
References
Nature. 2011 May 25;474(7350):173-8. doi: 10.1038/nature10143. Yeast. 2002 Jan 15;19(1):43-54. J Cell Biol. 2008 Sep 8;182(5):897-910. doi: 10.1083/jcb.200803098. Epub 2008 Sep 1.