Difference between revisions of "Part:BBa K1088009"

Revision as of 14:55, 22 August 2013

B. subtilis dxs-GFP protein fusion (lac promoter with LVA-tagged lac inhibitor (LacI:LVA) - IPTG ind

This is the same part as BBa_K1088008, with the exception of LacI under the control of a constitutive promoter to ensure regulation of the Lac Promoter This part consists of 9 subparts:

A constitutive promoter: BBa_J23106 A strong RBS: BBa_B0030 The LacI repressor protein, regulating the lac promoter: BBa_C0012 An artificial terminator: BBa_B1002 and the rest is similar to BBa_K1088008

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 2505
Illegal EcoRI site found at 3162
Illegal PstI site found at 2563
Illegal PstI site found at 3009
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 2505
Illegal EcoRI site found at 3162
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal PstI site found at 2563
Illegal PstI site found at 3009
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 2505
Illegal EcoRI site found at 3162
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 2505
Illegal EcoRI site found at 3162
Illegal PstI site found at 2563
Illegal PstI site found at 3009
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 2505
Illegal EcoRI site found at 3162
Illegal PstI site found at 2563
Illegal PstI site found at 3009
Illegal NgoMIV site found at 2462
Illegal AgeI site found at 2355
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2304
Illegal BsaI.rc site found at 4018
Illegal SapI.rc site found at 3003

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1088009 short</partinfo>
 This is the same part as BBa_K1088008, with the exception of LacI under the control of a constitutive promoter to ensure regulation of the Lac Promoter
-This part consists of 7 subparts:
+This part consists of 9 subparts:
 A constitutive promoter: BBa_J23106